Translational_Unit

Part:BBa_K622000:Design

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-01)


rpoN: coding sigma 54 factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 591
    Illegal BamHI site found at 554
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 250


Design Notes

Original CDS of rpoN has one restriction site of Pstl. We removed it by iPCR.


Source

Genomic DNA of E.coli

References